Två innehåller M. tuberculosis-specifika peptidantigener (TB1 TB2), ett innehåller kontrollmitogen (Mitogen) och ett innehåller inga stimulanter (Nil). Laboratoriet betraktar resultatet av IGRA-testet som positivt om resultatet av TB1 eller TB2 är 

All indeterminate – low mitogen (most cases) or nil > 8 IU/ml. • Negative result with high nil. • Negative result when the (TB Ag – nil) value = 0.25 to 0.35.

01. QuantiFERON TB2 Ag Value. 10 samples from each subject (20 samples in total) into the relevant tubes (nil, TB1, TB2 and mitogen). Samples were well mixed and incubated at 37°C for  Collect. 1 mL into each of 4 (Nil, TB1, TB2 and Mitogen) QFT-Plus Blood Collection Tubes.

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Whole blood is collected using the Quantiferon®-Plus Collection kit. The 4 collection tubes must be at 17-25°C at the time of blood filling. Collect 1 mL in each tube: [one NIL (Grey top), one TB1 Ag (Green top), one TB2 Ag (Yellow top), one MIT (Lavender top)]. The black mark on the side of tubes indicates 1 mL fill volume.

Collect. 1 mL into each of 4 (Nil, TB1, TB2 and Mitogen) QFT-Plus Blood Collection Tubes. Draw 1 ml into each tube and fill exactly up to the black line 

Custom QFT-Plus tubes: – Nil – same grey color. – TB1 – green, CD4 only. – TB2 – yellow: optimized to detect both CD4 and CD8! – Mitogen – same purple.

200 QFT-Plus HA Blood Collection Tubes (50 each: Nil, TB1, TB2 and Mitogen tubes) QFT-Plus is an in vitro diagnostic aid for detection of Mycobacterium tuberculosis infection. QFT-Plus is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography, and other medical and diagnostic evaluations.

The QFT-Plus Nil tube contains no antigens and is used to adjust for pre-existing IFN-γ (i.e., The IFN-γ level of the Nil tube sample is subtracted from the IFN-γ levels of the TB1, TB2, and Mitogen tube samples).

Both tubes contain peptide antigens from ESAT-6 and CFP-10 for stimulation of a CD4+ T-cell IFN-gamma response. In the 4 groups, 159 patients were positive by both TB1 and TB2 tubes, 1 patient was positive by TB1 only, and 4 were positive by TB2 only. A total of 1.1% (2/174) of results were indeterminate. The TB2 correctly identified 1 culture–confirmed patient that would have been indeterminate (low Mitogen) by TB1 result alone (see Tables 5 and 6 Der anvendes 4 prøverør per prøve: 1) negativ kontrol "QuantiFERON Nil" (gråt låg), to glas med TB specifikke antigener: 2)"QuantiFERON TB1" (grønt låg) og 3) "QuantiFERON TB2" (gult låg) og en postiv kontrol "QuantiFERON Mitogen" (lilla låg) Blod (lymfocytter) fra patienter stimuleres in vitro med specifikke antigener (ESAT6, CFP10), der er indeholdt i TB-antigen (Nil, TB1, TB2, Mitogen) 1x aiguille 21G 1½ pouce (0,8 x 38 mm) 1x porte-tubes de sécurité QUICKSHIELD 1x notice de test QuantiFERON®-TB Gold Plus (QFT®-Plus) Tubes de prélèvement sanguin Pack pour patient unique (N° de réf. 622222) À utiliser entre le niveau de la mer et 810 m d’altitude トロール（nil）と陽性コントロール（mitogen）があり， その他にtb1チューブとtb2チューブがある。tb1チ ューブはqft-3gと同様にcd4を刺激する抗原（esat-6 とcfp-10）が，tb2チューブにはcd4およびcd8を刺 激する刺激抗原（esat-6とcfp-10および短鎖cfp-10） Näytteenottojärjestys: Nil (harmaa), TB1 (vihreä), TB2 (keltainen), Mitogen (violetti).
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Collect 1 mL of blood into Nil, TB1, TB2 and Mitogen tubes (fill to blank line). Note : If butterfly needle is used, a purge tube must be collected first. 2.

• Yellow Top Tube (TB2 ANTIGEN). Mar 14, 2019 the Nil value from either TB antigen (Ag) tube. The mitogen tube serves as a control for the test.
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Lila kork med vit ring, Mitogen Control (stimuleringskontroll) Mätvärde för både TB1-NIL och TB2-NIL och ett sammanfattande resultat anges i 

The NIL tube value indicates if the patient has an ongoing immune response which can cause a false-positive result. Taking it step by step, first evaluate the Nil and the mitogen result and then the results of the TB1 and TB2. Significance of the NIL result: The NIL indicates the presence of any residual interferon-γ found in the patient’s blood due to an infection with an organism requiring cell mediated immunity/T cell immunity (e.g.

2019-3-26 · NIL 0.07 IU/mL KS MITOGEN-NIL >10.00 IU/mL KS TB-NIL 0.00 IU/mL KS The Nil tube value is used to determine if the patient has a preexisting immune response which could cause a false-positive reading on the test. In order for a test to be valid, the Nil tube must have a value of less than or equal to 8.0 IU/mL.

The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. 2018-01-22 · Nil TB1 ‐Nil TB2 ‐Nil Mitogen ‐Nil QualitativeResult Interpretation ≤8.0 IU/mL ≥0.35 and ≥25% of Nil Any Any Positive M.tuberculosis infection likely Any ≥0.35 IU/mLand ≥25% of Nil <0.35 IU/mL OR ≥0.35 IU/mL and <25% of Nil ≥0.5 IU/mL Negative M.tuberculosis infection NOT likely <0.5 IU/mL Indeterminate 2021-01-27 · The 4 collection tubes must be at 17-25°C at the time of blood filling.

Prov 2, grön kork och vit ring (TB1 antigen). Prov 3, gul kork och vit ring (TB2 antigen). Prov 4, lila kork och vit ring (Mitogen). Två innehåller M. tuberculosis-specifika peptidantigener (TB1 TB2), ett innehåller kontrollmitogen (Mitogen) och ett innehåller inga stimulanter (Nil). Laboratoriet betraktar resultatet av IGRA-testet som positivt om resultatet av TB1 eller TB2 är högre än referensvärdet och kontrollrören fungerar på godkänt sätt. Mitogen is a very insensitive marker, and only mitogen is slightly raised in your case.